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Zubeda Irshad
Rabbia Mahboob


Objectives: To determine the frequency of bacteria isolated from burn wounds, and their percent susceptibilities against a panel of antibiotics by analyzing burn wound culture, and susceptibility test results data of the patients managed in our burn center over a period of three years.
Methodology: In this descriptive, observational, cross-sectional, study a summary of antibiotic susceptibilities of isolated bacteria to tested antibiotics in percentages was prepared to assess the susceptibility pattern of bacteria. The prevalence of each of the isolated specie and genus of bacteria was determined. For susceptibility testing and antibiogram construction, the guide lines of Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. Supplement M100, and Analysis and Presentation of
Cumulative Antimicrobial Susceptibility Test Data; Approved Guideline—5th Edition: M39-2022 were followed. Excel and SPSS 24 were used for data entry and calculation of percentages and frequencies.
Results: Gram-negative bacteria were more commonly isolated as compared to gram-positive bacteria. Out of total 5166 isolated bacteria, 4334 (83.89 %) of isolates were gram-negative, whereas 832 (16 .11%) were gram-positive. The most commonly isolated bacteria were Klebsiella pneumoniae (K. pneumoniae) with 1095 (21.20%), followed by Pseudomonas aeruginosa (P. aeruginosa) with 989 (19.16%) occurrences and gram-positive cocci, respectively.
Conclusions: It is clear from our results that Gram-negative bacteria were more commonly involved in burn wound infection at our center. In addition, the isolated organisms had shown resistance to co-amoxiclav and cephalosporins and several other tested antibiotics.

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